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Growth (filled symbols) and β-galactosidase activity (open symbols) of KT2440 (circles), the fleQ mutant mus-69 (squares), and mus-69 with pMAMV1 (triangles) harboring the lapA::lacZ fusion in pMMGA (A) and the lapF::lacZ fusion in pMMG1 (B). Experiments were repeated three times with duplicate samples, and results of a representative experiment are shown. (C) Analysis of the relative expression (RE) of lapA and lapF in the fleQ mutant versus KT2440 by qRT-PCR.
