TABLE 1.
Strains and plasmids used in this work
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| P. putida strains | ||
| KT2440 | Wild type | Lab stock |
| mus-20 | lapF mutant (mini-Tn5 Km1) | 21 |
| mus-42 | lapA mutant (mini-Tn5 Km1) | 9 |
| mus-69 | fleQ mutant (mini-Tn5 Km1) | 9 |
| MM2 | gacS mutant (mini-Tn5 luxCDABE Km) | This work |
| C1R1 | rpoS null mutant | 27 |
| E. coli strains | ||
| DH5α | λ− ϕ80dlacZΔM15 Δ(lacZYA-argF)U196 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | Lab stock |
| MKW909 | S17-1 λpir (pUT mini-Tn5 ′luxCDABE Km2) | 28 |
| Plasmids | ||
| pMMG1 | lapF::lacZ transcriptional fusion, Tcr | 22 |
| pMMGA | lapA::lacZ transcriptional fusion, Tcr | 32 |
| pMMG2 | lapF::gfp transcriptional fusion, Gmr | 22 |
| pMMG5 | lapA::gfp transcriptional fusion, Gmr | This work |
| pMMG6 | lapA::mCherry transcriptional fusion, Tcr | This work |
| pMAMV1 | Diguanylate cyclase gene rup4959 in pBBR1MCS5, Gmr | 31 |
| pMAMV21 | rpoS::lacZ translational fusion in pMP220, Tcr | 31 |
| pMP220 | Promoter probe vector, promoterless lacZ, Tcr | 30 |
| pRU1097 | Promoter probe vector, promoterless gfp, Gmr | 29 |