TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| Strains | ||
| S. mutans | ||
| UA159 | Wild type, serotype c | 41 |
| IBSA99 | UA159 derivative, ΔSMU.662 Emr | This study |
| V403 | Wild type, serotype c | 75 |
| IBSA98 | V403 derivative, ΔSMU.662 Emr | This study |
| GS-5 | Wild type, serotype c, Smb producer | 39 |
| IBSA76 | GS-5 ΔsmbAB Emr, Smb nonproducer | 42 |
| GAS | ||
| JRS4 | Wild type, M6 serotype | 76 |
| IBSA66 | JRS4::ISS1 clone 1, insertion site not mapped, Emr | This study |
| IBSA67 | JRS4::ISS1 clone 4, insertion at codon position 8, Emr | This study |
| IBSA68 | JRS4::ISS1 clone 13, insertion at codon position 148, Emr | This study |
| IBSA70 | Plasmid-cured IBSA68, Ems | This study |
| Plasmids | ||
| pGEM-T Easy | Commercial TA cloning vector, Apr | Promega |
| pIB184-Km | E. coli-streptococcal shuttle plasmid, Kmr | 77 |
| pNM480 | E. coli vector for lacZ fusion, Apr | 55 |
| pIBM01 | pGEM-T Easy containing ermB gene, Apr Emr | 42 |
| pIBA35 | pIB184-Km containing SPy1384, Kmr | This study |
| pIBA39 | pNM480 with lacZ fused to SPy1384 at codon 75 (M75), Apr | This study |
| pIBA40 | pGEM-T Easy containing SPy1384, Apr | This study |
| pIBA41 | pNM480 with lacZ fused to SPy1384 at codon 149 (V149), Apr | This study |
| pIBA42 | pGEM-T Easy with the H178A mutation in SPy1384, Apr | This study |
| pIBA43 | pGEM-T Easy with the EE145/146AA mutations in SPy1384, Apr | This study |
| pIBA44 | pIB184-Km with the EE145/146AA mutations in SPy1384, Kmr | This study |
| pIBA45 | pIB184-Km with the H178A mutation in SPy1384, Kmr | This study |