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Purification and mass spectrometry of the VolA lipoprotein. (A) SDS-PAGE of purified VolA (∼82-kDa protein). (B) Mass spectrometry analysis showed a mature protein with a predominant mass of 83,521.0, a difference of 818.53 from the expected mass. (C) Predicted N-terminal modification of the VolA lipoprotein. Triacylation with two C16:0 and one C18:1 fatty acids (predicted mass, 818.77) would result in the observed mass of mature VolA. The lipid moiety is in red.
