TABLE 1.
S. aureus strains used in this study
| Strain | Phenotype, auxotrophism | Diagnosis | Patient treatment(s) | Gene with SNP | Nucleotide change | Amino acid change |
|---|---|---|---|---|---|---|
| LAC | Wild type, none | |||||
| 221SCV | SCV, menadione | Abscess | Clindamycin | menE | G762A | L254F |
| 221REV | Wild type | Wild-type menE | ||||
| 377SCV | SCV, menadione | Bacteremia | Vancomycin | |||
| Piperacillin | menC | G625A | A209V | |||
| Tazobactam | ||||||
| Gentamicin | menF | G382A | G128E | |||
| 377COM | Wild type | psk265menCa (wild type) | G625A | A209V | ||
| G382A | G128E | |||||
| 453SCV | SCV, menadione | Osteomyelitis | Vancomycin | menC | G514A | A172V |
| Nafcillin | ||||||
| Gentamicin | ||||||
| 453COM | Wild type | psk265menC (wild type) | G514A | A172V | ||
| 726SCV | SCV, menadione | Osteomyelitis | Ciprofloxacin, clindamycin | menC | G625A | A209V |
| menF | G382A | G128E | ||||
| 726COM | Wild type | psk265menC (wild type) | G625A | A209V | ||
| G382A | G128E | |||||
| 927SCV | SCV, menadione | Abscess | Clindamycin, trimethoprim-sulfamethoxazole | menF | A594G | I198M |
| 927REV | Wild-type menF |
a
psk265 contains the wild-type menC allele from strain LAC (menC accession number SAU300_1735 from the NCBI whole-genome database).