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(A) Motility analysis of 15 isolates of S. Dublin obtained from cattle. An SDu5 derivative fliC knockout mutant (fliC::kan) was included as a negative control. (B) Western blotting of total protein extracts from the 10 nonmotile S. Dublin isolates obtained from cattle, using a specific anti-FliC antibody. Detection of DnaK was used to verify equal loading of samples. Sizes of molecular mass markers are indicated in kDa.
