FIG 2.
Domain analysis of effects of oxygen on ToxR-TcpP interactions. (A) Full-length ToxR interacts with various TcpP truncations and chimeras. (B) Full-length TcpP interacts with various ToxR truncations and chimeras. The BTH101 strains containing those pairs were grown aerobically (gray bars) or anaerobically (white bars) at 37°C to an OD600 of ∼0.3, and β-galactosidase activity was measured and recorded in Miller units (29). β-gal, β-galactosidase; PP, periplasm; IM, inner membrane; CP, cytoplasm. White symbols, TcpP (P) domains; shaded symbols, ToxR (R) domains.