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ToxR-TcpP interactions in V. cholerae. The ΔtoxRS ΔtcpPH mutant harboring Plac-toxRS or PBAD-tcpPH was grown aerobically or anaerobically in AKI medium at 37°C and induced with 0.01% arabinose and 1 μM IPTG. Cell pellets were collected and resuspended in SDS-PAGE sampling buffer with or without DTT. Samples containing 0.1 μg total proteins were loaded onto a 15% polyacrylamide gel and subjected to Western blot analysis with anti-ToxR antibody (A) or anti-TcpP antibody (B).
