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Primers used for cloning
| Primer | Sequence (5′–3′)a |
|---|---|
| F- DnaJ-N | GGAATTCCATATGAACAATACTGAATTT |
| R-DnaJ-N | CGAGCTCTTCTCCATCAAAGG |
| F-DnaJ-C | CGGCGGCCGCATGAACAATACTGAATT |
| R-DnaJ-C | CCCTCGAGTTATTCTCCATCAAAGGCA |
| F-ΔA146Ply-N | GGAATTCCATATGGCA AAT AAAGCAGTA AAT |
| R-ΔA146Ply-N | CGAGCTCGTCATTTTCTACCTTATCCTCT |
| F-ΔA146Ply-C | CGGCGGCCGC ATGGCAAATAAAGCAGTAAATG |
| R-ΔA146Ply-C | CCCTCGAGTTACTAGTCATTTTCTACCTTATCCTCT |
Underlining indicates the recognition sites of restriction enzymes.
