FIG 2.

Functional complementation of E. coli and S. enterica mutants in waa genes with Francisella tularensis genes. (A) E. coli mutants in waaB, waaI, and waaJ from the Keio collection were complemented with a plasmid carrying either FTT1236 (pSL129) or FTT1237 (pSL30). Proteinase K-treated whole-cell lysates were prepared for each strain, separated by PAGE, and visualized by silver staining. *, upward mobility shift of lipid A-core molecules in each E. coli strain. (B) Whole-cell lysates (proteinase K treated) of S. enterica SL1344, the S. enterica waaL mutant, and the S. enterica waaL mutant complemented with FTT1238c (pSL149) were separated by PAGE, and O-acylated species were visualized by silver staining.