FIG 7.

Comparison of necrotic debris for Schu S4, Francisella tularensis waaY and waaL mutants, and complemented mutants. Groups of 5 BALB/c mice were infected with 33 CFU of Francisella tularensis Schu S4, 1.4 × 10⁶ CFU of F. tularensis waaY::TrgTn, 1.4 × 10⁶ CFU of F. tularensis waaL::TrgTn, 27 CFU of F. tularensis waaY::TrgTn plus pSL129, or 45 CFU of F. tularensis waaL::TrgTn plus pSL149. On day 4 postinfection, lungs were scored for percent airspace filled with necrotic debris. 0, no detected necropurulent debris in the airspaces; 1, 0 to 25% of airspaces partially to fully obstructed by necrotic cellular debris; 2, 25 to 50% obstructed; 3, 50 to 75% obstructed; 4, >75% obstructed. *, P < 0.01; **, P < 0.05; NS, not significant. P values were determined using Prism v5 software, Kruskal-Wallis one-way ANOVA, and the Dunn posttest.