TABLE 1.
Identification of F. tularensis Schu S4 genes involved in capsule productiona
| Gene locus | Tn5 insertion site (nucleotide position in gene) | Predicted function of gene product |
|---|---|---|
| FTT0025c (fslE) | 137 | Iron acquisition protein |
| FTT0846 | 896 | Potential deoxyribodipyrimidine photolyase |
| FTT1138 (hemH) | 637 | Potential ferrochelatase |
| FTT1236 | 688 | Hypothetical protein |
| FTT1238c | 463, 681, 989 | Hypothetical protein |
| FTT1458c (wzy) | 593 | O-antigen polymerase |
| FTT1464c (wbtA) | 641 | dTDP-glucose 4,6-dehydratase |
a
Identified by reduced binding of the gene mutants to anti-capsule antibody; all mutants had binding that was less than 15% of the WT level.