FIG 4.
MV stimulate IL-10 and IL-6 production from PBMCs. (A) PBMCs from healthy donors of unknown H. pylori infection status were incubated for 48 h in the presence of 0 to 12.5 μg/ml SS1WT or 60190 MV, and then IL-10 (left) and IL-6 (right) concentrations in culture supernatants were measured by ELISA. Similar data were obtained from independent experiments using PBMCs from three different donors; representative data from one donor are shown. (B) PBMCs from H. pylori-infected (closed circles) and uninfected (open circles) patients were incubated for 24 h in the presence of 10 μg/ml MV, 5 μg/ml concanavalin A, or an equivalent volume of PBS, then IL-10 (left) and IL-6 (right) concentrations in culture supernatants were measured by ELISA. NS, not significant.