FIG 1.

Identification of laminin and CK8 as a potential receptor for EAEC. T84 cells were incubated with 5 μg/ml of AafA-dsc protein or PBS (control), and bound AafA protein was cross-linked to T84 surface-exposed proteins with 1 mM DTSSP cross-linker (Pierce). Then, cells were lysed and cellular extracts were incubated with anti-AafA serum for immunoprecipitation analyses using A/G agarose columns. Immunoprecipitated proteins were visualized by silver-stained SDS-PAGE analyses, and protein bands were excised from the SDS gel for MALDI-TOF analyses.