FIG 2.

EAEC 042 binding to CK8 is dependent of AAF/II fimbriae. (A) EAEC 042 and its aafA and aafB mutants were added to 24-well plates coated with 5 μg/ml of CK8 or BSA, and the levels of bacteria bound were determined by plating. (B to D) EAEC strain 042 (B), AAF/II purified fimbriae (C), or AafA-dsc protein (D) was added to CK8- and BSA-coated wells, and binding was detected by ELISA. The bars represent the means of the results from three experiments, with the error bars indicating standard deviations. *, significantly different from BSA-coated control (P < 0.05). **, significantly different from EAEC 042 and its aafB mutant binding to CK8 (P < 0.05). Lm, laminin.