FIG 4.

ck8 gene knockdown reduces EAEC binding to epithelial cells. HEp-2 cells treated with scrambled or CK8 siRNA (A and C) and MDCK cells (C) were infected by EAEC 042 or EAEC 042aafA. Numbers of adherent bacteria were determined by counting of the CFU. *, significantly different from adhesion to HEp-2 with the scrambled siRNA (P < 0.05). (B) Immunofluorescence of HEp-2 cells treated with scrambled or CK8 siRNA and infected with EAEC 042. Actin was visualized in red (TRITC-phalloidin), and bacterial or human DNA was visualized in blue (TO-PRO-3).