FIG 2.

Assessment of the efficiency of neutrophil depletion in mice treated with monoclonal antibody RB6-8C5 or 1A8. Neutrophil depletion and sample preparation are described in Materials and Methods. (A to D) FACS analyses of Ly6G⁺/CD11b⁺ neutrophils among all white cells from mice that were treated with DPBS (A), RB6-8C5 (B), 1A8 (C), or 2A3 (D). (E) Percentages of neutrophils and macrophages at the skin infection site of MGAS2221 at 24 h after inoculation. The numbers of neutrophils and macrophages were counted among 150 cells by examining H&E-stained skin samples under a microscope. (F) Levels of infiltrated neutrophils at the infection site in the skin at 24 h after inoculation of 9.4 × 10⁷ CFU MGAS2221 in C57BL/6 mice. The mice were intraperitoneally injected with DPBS, RB6-8C5, or 1A8 at 24 h prior to MGAS2221 infection. PMN, polymorphonuclear leukocytes.