TABLE 1.
Straina | covRS mutation | Mutated CovS or CovR | SpeB production | In vitro SsEb production |
---|---|---|---|---|
1 | 132TTTCT136 deletion in covS | Truncated CovS | − | + |
2 | 223G to T in covS | Truncated CovS | − | + |
3 | 1166C to A in covS | CovSAla388Asp | − | + |
4 | 481C to T in covS | Truncated CovS | − | + |
5 | Insertion of ATTTTCTCTGC at nucleotide 99 in covS | Truncated CovS | − | + |
6 | 616A to T in covR | CovRIle205Phe | − | + |
7 | 616A to T in covR | CovRIle205Phe | − | + |
8 | 1166C to A in covS | CovSAla388Asp | − | + |
9 | 1363C to T in covS | Truncated CovS | − | + |
10 | 1363C to T in covS | Truncated CovS | − | + |
11 | 1166C to A in covS | CovSAla388Asp | − | + |
12 | None | wt | + | − |
13 | None | wt | + | − |
14 | wt | wt | + | − |
Strains 1 through 11 were all SpeBA− variants detected among 48 randomly chosen colonies isolated from a mouse infected with MGAS2221, and strains 12 and 13 were randomly chosen from 37 SpeBA+ colonies in the same test. Strain 14 was MGAS2221.
The SsE production status was assigned according to the data shown in Fig. 7.
wt, wild type.