FIG 5.

Contribution of SrrAB and AgrAC TCSs to plc regulation. The relative expression of plc was examined in an S. aureus USA300 ΔsrrAB background (A) or a Δagr background (C) after 60 min of exposure to PBS or various concentrations of HOCl. In addition, the relative expression of plc in PMNs at 10 min postphagocytosis was examined in an S. aureus USA300 ΔsrrAB background (B) or Δagr background (D). Controls included broth-grown bacteria and bacteria that have been opsonized for 20 min (Ops). The level of plc expression was normalized to that of the DNA gyrase subunit B (gyrB), and results represent the means ± SEMs from three independent experiments. **, P < 0.01.