TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description or function | Source or reference |
|---|---|---|
| Strains | ||
| E. coli strains | ||
| DH5α | Plasmid maintenance | Protein Express |
| TOP10 | Cloning strain | Invitrogen |
| BL21 Star(DE3) | Recombinant protein production | Invitrogen |
| S. aureus strains | ||
| AH1263 | USA300 CA-MRSA Erms (LAC*) | 55 |
| RN4220 | Restriction deficient | 56 |
| MRSA252 | USA200 MRSA | 57 |
| UAMS-1 | Osteomyelitis isolate | 58 |
| Newman | MSSA | 59 |
| COL | HA-MRSA | 60 |
| MW2 | USA400 CA-MRSA | 61 |
| NRS22 | USA600 MRSA | 62 |
| TCH1516 | USA300 CA-MRSA | 63 |
| JMB1145 | LAC* SAUSA300_2337-38Δ | This work |
| JMB1148 | LAC* SAUSA300_0217-18Δ | This work |
| JMB1219 | LAC* SAUSA300_1219-20Δ | This work |
| JMB1223 | LAC* SAUSA300_2035-36Δ | This work |
| JMB1232 | LAC* SAUSA300_2558-59Δ | This work |
| JMB1241 | LAC* SAUSA300_1798-99Δ | This work |
| JMB1357 | LAC* SAUSA300_0254-55Δ | This work |
| JMB1358 | LAC* SAUSA300_1638-39Δ | This work |
| JMB1359 | LAC* SAUSA300_2308-09Δ | This work |
| JMB1377 | LAC* SAUSA300_1865-66Δ | This work |
| JMB1383 | LAC* SAUSA300_1307-08Δ | This work |
| JMB1293 | LAC* SAUSA300_1441-42Δ | This work |
| AH1292 | LAC* Δagr::tetM | 64 |
| AH1558 | LAC* sae::spec | 65 |
| JMB1515 | LAC* SAUSA300_0645-46Δ | This work |
| MJW44 | LAC* SAUSA300_0099Δ(plc) | This work |
| MJW120 | RN4220 Δplc | This work |
| MJW131 | Newman Δplc | This work |
| Plasmids | ||
| pJB38 | Mutation generation vector | 66 |
| pCM28 | Complementation vector | 30 |
| pET28a | Protein expression vector | Invitrogen |
| pSKerm-MCS | Complementation vector | 67 |
| pSK236 | Shuttle vector | 68 |
| pET16b | Protein expression vector | Invitrogen |
| pMW1 | pJB38 SAUSA300_0099Δ | This work |
| pMW4 | pSKerm-MCS SAUSA300_0099 | This work |
| pMW12 | pET28a containing srrA | This work |
| pMW14 | pSK236 containing scn promoter and plc | This work |
| pMW17 | pET16b containing srrB kinase domain | This work |
| pMW20 | pCM28 containing plc | This work |
| pCM29 | pCM28 containing sGFP | 30 |
| pCR2.1 | Cloning vector | Invitrogen |
| pJMB150 | pJB38 SAUSA300_0217-18Δ | This work |
| pJMB155 | pJB38 SAUSA300_2337-38Δ | This work |
| pJMB168 | pJB38 SAUSA300_2035-36Δ | This work |
| pJMB170 | pJB38 SAUSA300_1219-20Δ | This work |
| pJMB181 | pJB38 SAUSA300_2558-59Δ | This work |
| pJMB183 | pJB38 SAUSA300_1798-99Δ | This work |
| pJMB202 | pJB38 SAUSA300_1307-08Δ | This work |
| pJMB204 | pJB38 SAUSA300_1865-66Δ | This work |
| pJMB205 | pJB38 SAUSA300_2308-09Δ | This work |
| pJMB210 | pJB38 SAUSA300_0254-55Δ | This work |
| pJMB211 | pJB38 SAUSA300_1638-39Δ | This work |
| pJMB229 | pJB38 SAUSA300_1441-42Δ | This work |
| pJMB230 | pJB38 SAUSA300_0645-46Δ | This work |