FIG 4.

Imaging agent potency ELISA. MAbs underwent DOTA conjugation via NHS chemistry and subsequent mock labeling conditions without the addition of ⁶⁴Cu. To ascertain the effects of MAb labeling on the ability to bind selected targets, corresponding proteins were coated on ELISA wells and allowed to react with MAbs at the indicated concentrations. The bound MAbs were visualized with HRP-labeled secondary antibodies, followed by color development with TMB substrate. The results for triplicate wells were averaged and plotted. (A) MAb 69; (B) isotype-matched control MAb 7.