FIG 6.

IFN-γ production by memory phenotype T cells. WT and CARD9 KO mice were infected intratracheally with C. neoformans, and the lung leukocytes were prepared on day 3 postinfection. (A) Expression of IFN-γ in CD4⁺ or CD8⁺ naive and memory phenotype T cells, identified as CD44^dull+ and CD44^bright+ cells, was analyzed in a using flow cytometry. Representative histograms from three WT mice are shown. (B) The number of each subset expressing IFN-γ was calculated. Data are means and SD for three mice. Experiments were repeated twice with similar results. MPT cells, memory phenotype T cells. NS, not significant; *, P < 0.05.