TABLE 1.
Primer sequences for real-time PCR
| Gene product | Forward primer | Reverse primer |
|---|---|---|
| IFN-γ | ACTGCCACGGCACAGTCATT | TCACCATCCTTTTGCCAGTTCCT |
| TGF-β | TACGCCTGAGTGGCTGTCTTTT | CGTGGAGTTTGTTATCTTTGCTGT |
| IL-6 | CACGGCCTTCCCTACTTCACAA | TTGCACAACTCTTTTCTCATTTCCACG |
| IL-12p35 | GAGTTCCAGGCCATCAACGCA | GCTTCTCCCACAGGAGGTTTCTG |
| IL-17A | AACCGTTCCAC GTCACCCTG | GTCCAGCTTTCCCTCCGCAT |
| IL-23p19 | CTCAGCCAACTCCTCCAGCCAG | CTGCTCCGTGGGCAAAGACC |
| CCL3 | AACCAGCAGCCTTTGCTCCC | GGTCTCTTTGGAGTCAGCGCA |
| CCL4 | AAACCTAACCCCGAGCAACACC | GAAACAGCAGGAAGTGGGAGGG |
| CCL5 | ACTCCCTGCTGCTTTGCCTAC | GGCGGTTCCTTCGAGTGACAA |
| CXCL9 | GGCACGATCCACTACAAATCCCT | AGGCAGGTTTGATCTCCGTTCTTC |
| CXCL10 | GTGCTGCCGTCATTTTCTGCC | AGGATAGGCTCGCAGGGATGA |
| iNOS | AGGGAATCTTGGAGCGAGTGGT | GCAGCCTCTTGTCTTTGACCC |
| T-bet | CACTAAGCAAGGACGGCGAATG | TCCACCAAGACCACATCCACAA |
| ROR-γt | CTTCCTCAGCGCCCTGTGTT | CCCAGGACGGTTGGCATTGA |
| HPRT | GCTTCCTCCTCAGACCGCTT | TCGCTAATCACGACGCTGGG |