FIG 2.

In vitro antimicrobial activity assay. Diluted stocks of U. parvum or U. urealyticum were incubated with synthetic AMP or AMP solvents at the indicated concentrations for 30 min at 37°C. After treatment, bacteria were incubated with DiBAC₄, to label depolarized cells. Control viable nondepolarized bacteria from freshly thawed stock (low-fluorescence gate P5 [A]) and depolarized cells after treatment with LL-37 (depolarized high-fluorescence gate P6 [B]) are shown as representative examples. A total of 5,000 to 10,000 events were collected from each experimental treatment. The survival rates were calculated by normalizing the percentage of viable, nondepolarized cells in the experimental group to the value for the negative controls (C and D). Data represent average survival rates ± standard deviations from 3 experiments.