FIG 3.

Histone modification alterations in the defense gene promoters in Ureaplasma-infected THP-1 cells. THP-1 cells (1 × 10⁶) were cocultured with U. parvum or U. urealyticum (5 CFU/cell) or PMA (200 nM) for 16 h. Formaldehyde-cross-linked, sheared chromatin was immunoprecipitated using anti-acetyl-histone H3 (Lys9 [A]) or anti-trimethyl-histone H3 (Lys9 [B] or Lys4 [C]). Immunoprecipitated DNA fragments were quantified by qPCR using primers specific for the promoter regions of the genes tested. Histone modification alterations were expressed as the ratio of immunoprecipitated chromatin target (% input) from stimulated to unstimulated cells. Data are the average fold changes of histone modifications ± standard deviations of three independent experiments.