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. 2014 Apr;82(4):1657–1665. doi: 10.1128/IAI.01231-13

TABLE 1.

Primers for gene expression quantification, ChIP DNA quantification, and DNA methylation analysis

Gene Primer name Primer sequence (5′–3′) Application Source
ACP6 ACP6-eF ATAGCTGGGGGTTCCACTCT Gene expression quantification qPrimerDepot
ACP6-eR AAAATGGTGCAGGTCGTGTT
CAMP CAMP-eF GGGCACACTGTCTCCTTCAC Gene expression quantification qPrimerDepot
CAMP-eR TCGGATGCTAACCTCTACCG
CAT CAT-eF ACGGGGCCCTACTGTAATAA Gene expression quantification qPrimerDepot
CAT-eR AGATGCAGCACTGGAAGGAG
DEFA5 DEFA5-eF GGACTCACGGGTAGCACAAC Gene expression quantification qPrimerDepot
DEFA5-eR CCTTTGCAGGAAATGGACTC
DEFA6 DEFA6-eF GACCTTCTGCAATGGCAAGT Gene expression quantification qPrimerDepot
DEFA6-eR AGGACTTTGCCGTCTCCTTT
DEFB1 DEFB1-eF GGGCAGGCAGAATAGAGACA Gene expression quantification qPrimerDepot
DEFB1-eR TTTTGTCTGAGATGGCCTCA
IL-8 IL-8 F CTAGGACAAGAGCCAGGAAGA Gene expression quantification 49
IL-8 R AACCCTCTGCACCCAGTTTTC
MPO MPO-eF TCCCGAAGTAAGAGGGTGTG Gene expression quantification qPrimerDepot
MPO-eR CCCTGTCTCCTCACCAACC
RPLP0 RPLP0-F ATCTGCTTGGAGCCCACAT Gene expression quantification qPrimerDepot
RPLP0-R GCGACCTGGAAGTCCAACTA
TNFA TNFa1-F CCCAGGCAGTCAGATCATCTTC Gene expression quantification 50
TNFa1-R GGTTTGCTACAACATGGGCTACA
CAMP CAMP-F1 GGCTTGGGAACATTTTGAGA ChIP PCR This study
CAMP-R1 ATCCCCTTCTGCATCCTTCT
DEFA5 DEFA5-F1 GGAGCATCAAAGGGATCTTG ChIP PCR This study
DEFA5-R1 TGAGGAGTCAGCCTGGATTT
DEFA6 DEFA6-F1 AGCATCAAAGGGACATGGAG ChIP PCR This study
DEFA6-R1 AGGAGCCAGCCTGGATTTAT
DEFB103 DEFB103-F1 CAGTCTGGGCAGCATAGTGA ChIP PCR This study
DEFB103-R1 GCCTCCACACATGGCTAAAT
DEFB104 DEFB104-F1 AGAAACGATTCAGGGAAGCA ChIP PCR This study
DEFB104-R1 GAGGTGTTGGGGCTACAGAA
DEFB1 DEFB1-F1 TCCAGAAACCCCATCAGAAC ChIP PCR This study
DEFB1-R1 CCGCTGGATTTAGCTTTCAG
TNFA TNFA-F1 ACCACAGCAATGGGTAGGAG ChIP PCR This study
TNFA-R1 GTCCCCATACTCGACTTCCA
DEFA6 defa6_msF GGTTTTTATTAGGGTTTTTATGTGG DNA methylation This study
defa6_msR ACCCATTTACTCTAAAATCCAACTTT
DEFB1 defb1_msF1 TTAAGGAAAATTTGAGGGATATTTG DNA methylation This study
defb1_msR1 CTTCCTCTAACCAAAACTACCTTCTC
DEFB104A defb104a_msF2 GTGTTTTTTTTAAGGGTAAATGTTAGG DNA methylation This study
defb104a_msR2 AATAACAACAAAAAATACTTAATAAC
defb4_msF1 TGAGGATGGGGTTTATTATGTTTT
DEFB4 defb4_msR1 CCACTCACCTACTACCCACTCTATAC DNA methylation This study
defb4_msF2 TTTTGAAAATATGGGGTTTTTTTATAA
defb4_msR2 CACTCAAAAAATATCAACTCCACAA
TNFA TNFa_msF TTTATTTTTTTATTTAAGGGGAAATG DNA methylation This study
TNFa_msR CCATATACCAAACATCCTATCTCTC