TABLE 1.
Laboratory-constructed GAS strains used in this study
| Strain | Serotype | Information | Reference |
|---|---|---|---|
| 2221ΔfasC | M1 | Derivative of clinical isolate MGAS2221 in which the fasC gene has been replaced with a spectinomycin resistance cassette | This work |
| 2221ΔrivR | M1 | Derivative of clinical isolate MGAS2221 in which the rivR gene has been deleted via homologous recombination | 34 |
| 10870fasCComp | M3 | Derivative of clinical isolate MGAS10870 in which the 4-bp deletion naturally present in the fasC gene has been fixed via homologous recombination | This work |
| 10870ΔrivR | M3 | Derivative of clinical isolate MGAS10870 in which the rivR gene has been deleted via homologous recombination | This work |
| 10870rivR+1bp | M3 | Derivative of clinical isolate MGAS10870 in which the 1-bp deletion naturally present in the rivR gene has been fixed via homologous recombination | This work |
| 10870rivR+1bp fasCComp | M3 | Derivative of 10870fasCComp in which the 1-bp deletion naturally present in the rivR gene has been fixed via homologous recombination (hence has wild-type fasC and rivR alleles) | This work |
| 315fasCComp | M3 | Derivative of clinical isolate MGAS315 in which the mutation naturally present in fasC has been fixed via homologous recombination | This work |
| 315fasCMUT | M3 | Derivative of 315fasCComp in which the fasC gene has been remutated via homologous recombination to look like that present in clinical M3 isolates | This work |
| 2221 + vector | M1 | Derivative of clinical isolate MGAS2221 containing an empty derivative of shuttle vector pDC123 | This work |
| 2221 + pRivR-M1 | M1 | Derivative of clinical isolate MGAS2221 containing the pDC123 derivative pM1rivR, which contains the rivR gene from M1 GAS strain MGAS2221 | This work |
| 2221 + pRivR-M3 | M1 | Derivative of clinical isolate MGAS2221 containing the pDC123 derivative pM3rivR, which contains the rivR gene from M3 GAS strain MGAS10870 | This work |
| 2221ΔrivR + vector | M1 | Derivative of isolate 2221ΔrivR containing an empty derivative of shuttle vector pDC123 | This work |
| 2221ΔrivR + pRivR-M1 | M1 | Derivative of isolate 2221ΔrivR containing the pDC123 derivative pM1rivR, which contains the rivR gene from M1 GAS strain MGAS2221 | This work |
| 2221ΔrivR + pRivR-M3 | M1 | Derivative of isolate 2221ΔrivR containing the pDC123 derivative pM3rivR, which contains the rivR gene from M3 GAS strain MGAS10870 | This work |
| 10870 + vector | M3 | Derivative of clinical isolate MGAS10870 containing an empty derivative of shuttle vector pDC123 | This work |
| 10870 + pRivR-M1 | M3 | Derivative of clinical isolate MGAS10870 containing the pDC123 derivative pM1rivR, which contains the rivR gene from M1 GAS strain MGAS2221 | This work |
| 10870 + pRivR-M3 | M3 | Derivative of clinical isolate MGAS10870 containing the pDC123 derivative pM3rivR, which contains the rivR gene from M3 GAS strain MGAS10870 | This work |
| 10870ΔrivR + vector | M3 | Derivative of isolate 10870ΔrivR containing an empty derivative of shuttle vector pDC123 | This work |
| 10870ΔrivR + pRivR-M1 | M3 | Derivative of isolate 10870ΔrivR containing the pDC123 derivative pM1rivR, which contains the rivR gene from M1 GAS strain MGAS2221 | This work |
| 10870ΔrivR + pRivR-M3 | M3 | Derivative of isolate 10870ΔrivR containing the pDC123 derivative pM3rivR, which contains the rivR gene from M3 GAS strain MGAS10870 | This work |
| 10870 + pFasX | M3 | Derivative of clinical isolate MGAS10870 containing the pDC123 derivative pFasX, which expresses the wild-type fasX gene | This work |
| 315 + vector | M3 | Derivative of clinical isolate MGAS315 containing an empty derivative of shuttle vector pDC123 | This work |
| 315 + pFasX | M3 | Derivative of clinical isolate MGAS315 containing the pDC123 derivative pFasX, which expresses the wild-type fasX gene | This work |