TABLE 1.
Primer sets used for qRT-PCR for HT-29 cells
| Gene name | Accession no. | Product size (bp) | Direction | Sequence (5′→3′) |
|---|---|---|---|---|
| IL-1β | NM_000576 | 126 | Forward | GTGGCAATGAGGATGACTTGT |
| Reverse | TGTAGTGGTGGTCGGAGATTC | |||
| IL-8 | NM_000584 | 88 | Forward | GAGGGTTGTGGAGAAGTTTTTG |
| Reverse | CTGGCATCTTCACTGATTCTTG | |||
| IL-18 | NM_001562 | 100 | Forward | TGAAGATGATGAAAACCTGGAAT |
| Reverse | TTGGTCAATGAAGAGAACTTGGT | |||
| MUC1 | NM_001018017.2 | 108 | Forward | CAGCCAGCGCCTGCCTGAAT |
| Reverse | GCACTGTGAGGAGCAGCAGCA | |||
| MUC2 | M94132.1 | 138 | Forward | CCTGCCGACACCTGCTGCAA |
| Reverse | ACACCAGTAGAAGGGACAGCACCT | |||
| MUC3 | AB038784.1 | 130 | Forward | CTCGTGTTGCCATTGCCTCTCTCG |
| Reverse | CTGCAGGTTGCCTCCAGGTTCAGA | |||
| TNF-α | AF043342 | 100 | Forward | AACCTCCTCTCTGCCATCAA |
| Reverse | GGAAGACCCCTCCCAGATAG | |||
| gapdh | NM_002046 | 94 | Forward | ACAGTCAGCCGCATCTTCTT |
| Reverse | ACGACCAAATCCGTTGACTC |