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M. tuberculosis bacilli are associated with cultured neurons. Neuronal cultures—HT22, Neuro-2a, and primary neurons—established from the hippocampi of 1-day-old C57BL/6 neonates were infected with M. tuberculosis at an MOI of 30:1 for 6 and 24 h. Ziehl-Neelsen staining shows the presence of acid-fast bacilli (arrows) in the cytoplasm and neurites of neurons. Scale bars, 20 μm.
