FIG 6.

Confocal microscopy of internalized M. tuberculosis bacilli in human SK-N-SH cultured neurons. (B, C, E, and F) SK-N-SH cultured neurons labeled with anti-β-III-tubulin (B and C) and anti-MAP2 (E and F) antibodies (red). (B and E) Maximum-intensity projections of z-stacks of entire cells. (C and F) Orthogonal projections representing 3-dimensional data sets provide a view of defined optical sections in the x-y, as well as the z, dimensions of the original z-stack. Cell nuclei are labeled with DAPI (blue). (A and D) DIC images showing the tissue localization of H37Rv-GFP bacilli (green). Scale bar, 20 μM. The images represent neuronal cultures at 48 h.