TABLE 1.

Proteins identified by LC-MS/MS according to biological process, localization, and pattern of expression profile

Category and spot no.a	Protein name	Accession no.	Localization	Scoreb	Coveragec (%)	No. of peptides matchedd	pI	Nominal mass	Change in expression ate:
									6 h	12 h	24 h
Immune response
6 h, no. 6	Annexin A1	NP_000691	Plasma membrane	516	33	11	6.57	38,918	Down	Up	Up
12 h, no. 15				651	38	11	6.57	38,918
24 h, no. 13				557	39	13	6.57	38,918
24 h, no. 7	Leukotriene A4 Hydrolase	1GW6_A	Cytoplasm	340	18	9	5.87	69,736	—	—	Down
6 h, no. 13	Moesin	EAX05399	Cytoplasm	360	20	12	5.9	66,678	Down	—	Down
24 h, no. 5				202	11	9	6.08	67,892
12 h, no. 21	Protein DJ-1	NP_009193	Cytoplasm	79	25	5	6.33	20,050	—	Down	—
24 h, no. 9	Tapasin	3F8U_A	Endoplasmic reticulum	454	23	11	5.61	54,541	—	—	Down
6 h, no. 5	T-cell receptor β chain	AAC96236	Plasma membrane	55	34	4	12.22	4,975	Up	—	—
Metabolism
24 h, no. 1	Alpha glucosidase II α subunit	AAH65266	Endoplasmic reticulum	69	3	5	5.36	96,475	—	—	Down
12 h, no. 13	Aryl hydrocarbon receptor nuclear translocator	CAB65056	Nucleus	62	89	5	9.97	2,403	—	Down	—
6 h, no. 7	ATP synthase β	ABD77240	Mitochondrion	849	45	25	4.95	48,083	Down	—	—
6 h, no. 4	Calreticulin	BAD96780	Endoplasmic reticulum	103	9	6	4.3	47,061	Up	—	—
6 h, no. 18	CCT8 protein	AAH12584	Cytoplasm	115	11	7	5.18	54,585	Down	—	—
6 h, no. 14	Elongation factor Tu	AAB00499	Mitochondrion	308	17	7	7.7	49,851	Down	—	—
6 h, no. 2	Enolase 1	NP_001419	Cytoplasm	504	27	13	7.01	47,481	Down	Up	Up
12 h, no. 10				734	35	17	7.01	47,481
24 h, no. 10				487	23	9	7.01	47,481
12 h, no. 20	Glutathione Transferase P1-1	1EOH_A	Cytoplasm	441	48	12	5.74	23,394	—	Up	—
12 h, no. 23	l-Lactate dehydrogenase	NP_002291	Cytoplasm	339	21	11	5.71	36,900	—	Down	—
6 h, no. 16	Phosphoglycerate mutase 1	AAH62302	Cytoplasm	290	41	8	6.67	28,916	Down	Up	Up
12 h, no. 19				120	21	3	6.67	28,916
24 h, no. 17				109	21	3	6.67	28,916
6 h, no. 8	Proteasome activator complex subunit 1	NP_006254	Cytoplasm	378	42	13	5.78	28,876	Up	—	—
24 h, no. 19	Superoxide dismutase 2	AAP34408	Mitochondrion	227	22	5	6.87	23,324	—	—	Up
6 h, no. 24	T-complex protein 1 subunit β isoform 1	NP_006422	Cytoplasm	334	19	13	6.01	57,794	Down	Down	Down
12 h, no. 4				351	17	9	6.10	61,066
24 h, no. 8				317	17	11	6.03	60,869
24 h, no. 18	Triosephosphate isomerase 1	NP_000356	Cytoplasm	405	48	13	6.45	26,938	—	—	Up
Signal transduction
6 h, no. 23	Annexin A2	NP_004030	Nucleus	484	31	9	7.57	38,808	Down	Up	Up
12 h, no. 16				518	24	9	7.57	38,808
24 h, no. 12				578	28	9	7.57	38,808
12 h, no. 18	Annexin A4	BAD92694	Nucleus	248	21	7	6.03	25,633	—	Down	—
24 h, no. 14	Annexin A5	NP_001145	Cytoplasm	408	28	13	4.94	35,971	—	—	Up
6 h, no. 12	CAPf	EAX07240	Cytoplasm	126	8	4	7.64	51,357	Down	—	—
6 h, no. 15	Discoidin domain receptor 2	AAH02433	Plasma membrane	282	24	10	8.02	50,335	Up	—	—
12 h, no. 5	Hsp70/Hsp90 protein	XP_001691869	Nucleus	207	11	10	7.81	68,721	—	Up	—
12 h, no. 14	Lim and SH3 protein 1	AAH12460	Cytoplasm	111	17	10	8.06	30,038	—	Down	—
12 h, no. 9	Rho GTPase-activating protein 1	NP_004299	Cytoplasm	412	31	3	6.11	51,087	—	Down	—
Stress response
6 h, no. 17	Heat shock protein 90	AAI08696	Cytoplasm	474	24	21	5.11	68,614	Up	—	—
24 h, no. 2	Tumor rejection antigen 1	NP_003290	Endoplasmic reticulum	571	16	15	4.76	92,696	—	—	Down
Structure
6 h, no. 1	Actin-related protein 3	NP_005712	Cytoplasm	308	19	12	5.61	47,797	Down	—	—
6 h, no. 11	Ezrin	NP_003370	Cytoplasm	456	16	13	5.94	69,484	Down	Down	Down
12 h, no. 2				493	20	15	5.94	69,484
24 h, no. 4				421	15	11	5.94	69,484
6 h, no. 9	F-actin-capping β subunit	NP_004921	Cytoplasm	286	26	7	5.69	30,952	Up	—	—
6 h, no. 22	Gelsolin	NP_937895	Cytoplasm	74	9	3	5.58	80,876	Down	Down	Down
12 h, no. 1				165	7	11	5.58	80,876
24 h, no. 3				208	9	11	5.58	80,876
6 h, no. 3	Tropomyosin 3	NP_705935	Cytoplasm	221	18	7	4.70	28,800	Up	—	Down
24 h, no. 20				129	18	7	4.75	29,243
12 h, no. 8	Vinculin	NP_003364	Cytoplasm	441	13	16	5.83	117,220	—	Down	—
6 h, no. 10	WDR1 protein	AAD05045	Cytoplasm	268	16	6	6.41	58,593	Down	Down	—
12 h, no. 3				297	19	10	6.41	58,593
Cell cycle
12 h, no. 6	Annexin A11	NP_001148	Cytoplasm	387	16	8	7.53	54,697	—	Down	—
6 h, no. 20	Septin 6	NP_665798	Nucleus	337	26	15	6.35	49,184	Down	Down	—
12 h, no. 22				129	7	5	6.35	49,184
Transcription
6 h, no. 21	LIM domain binding	AAC05580	Cytoplasm	265	18	7	6.8	36,604	Up	—	Up
24 h, no. 16				210	15	7	6.8	36,604
Translation
12 h, no. 12	DEAD box protein 48	NP_055555	Nucleus	268	29	12	6.3	47,126	—	Down	—
6 h, no. 19	Pre-mRNA-processing factor 19	NP_055317	Nucleus	115	9	6	6.14	55,603	Down	Down	—
12 h, no. 7				141	14	6	6.14	55,603
24 h, no. 11	Ribosomal protein L4	EAW77773	Ribosome	77	15	8	11.03	37,861	—	—	Down
Transport
24 h, no. 6	Annexin A6	NP_001180473	Cytoplasm	606	33	23	5.42	72,720	—	—	Down
24 h, no. 15	Chloride intracellular channel 1	NP_001279	Nucleus	337	26	9	5.02	27,249	—	—	Down
24 h, no. 21	Ferritin heavy chain 1	EAW73993	Cytoplasm	70	15	4	6.04	26,374	—	—	Up
12 h, no. 11	GDP dissociation inhibitor 2	NP_001485	Cytoplasm	412	31	15	6.11	51,087	—	Down	—
Unknown
12 h, no. 17	Glyoxalase domain-containing protein 4	NP_057164	Mitochondrion	142	12	4	5.4	33,554	—	Down	—

^aThe spot numbers correspond to the numbers on the 2D gels in Fig. 2.

^bProbability score in Mascot program (a Mascot score of >55 corresponds to a probability of <5% that the observed match is a random event).

^cPercent coverage of the matched peptide in relation to the full-length sequence.

^dNumber of matched peptides in database search.

^eDown, significantly downregulated (P < 0.05); up, significantly upregulated (P < 0.05); —, no significantly change in expression compared with control.

^fAdenylate cyclase-associated protein.