FIG 2.

IL-17A responses and protection against colonization following intranasal immunization with lipidated or nonlipidated SP_2108 and SP_0148. (A) C57BL/6J mice were immunized once and then again 1 week later with 4 μg of the indicated protein and 1 μg of CT in a 10-μl volume instilled intranasally. Two weeks following their last immunization, animals were bled and whole blood was stimulated with WCA; IL-17A concentrations in cell supernatants following a 6-day incubation are shown. (B) One week following intranasal challenge, nasal washes were collected and pneumococcal CFU were counted. Bars represent median values, and P values were calculated by Kruskal-Wallis analysis of comparisons between CT, SP_21208, and SP_2108Δlipid groups (solid bars) and between CT, SP_0148, and SP_0148Δlipid groups (dashed bars).