In vitro proinflammatory responses to lipidated and nonlipidated proteins. WT or TLR2−/− macrophages from C57BL/6 mice were stimulated with lipidated or nonlipidated proteins, a fusion of SP_2108 and SP_1912, or Pam3CSK4, all at 1 μg/ml. LPS was used at 100 ng/ml. Values shown represent at least 4 independent stimulations harvested at 3, 6, and 24 h poststimulation. Bars and lines represent medians with interquartile ranges.