FIG 4.

Immunization with lipoprotein-based subunit vaccine in WT and Tlr2^−/− mice. WT (C57B6/J) or Tlr2^−/− (C57B6.129-Tlr2^tm1Kir/J) mice (n = 10 to 20/group) were immunized subcutaneously three times with alum only, 1 μg of SP_2108 and 10 μg each of SP_0148 and SP_1912 absorbed on alum, or a fusion protein of SP_2108 and SP_1912 adsorbed on alum. (A) IL-17A concentrations in cell supernatants of whole blood following a 6-day stimulation with WCA are shown. (B) Ten days following intranasal challenge, nasal washes were collected and pneumococcal CFU were counted. Bars represent medians, and P values were calculated using the Mann-Whitney test. NS, not significant.