FIG 3.

Antibiotic treatment for 4 or 6 days decreased the expression of nondefensin proteins of the intestinal mucosa. Expression of RegIIIβ, RegIIIγ, CRP-ductin, RELMβ, Crypt1, Crypt4, Defcr-rs-10, TLR4, and TLR2 mRNAs in the terminal ileum was analyzed by real-time PCR (A) and RT-PCR (B). (C) Protein expression of RegIIIβ, RELMβ, CRP-ductin, TLR4, MyD88, and α-defensin 4 in the terminal ileum was analyzed by immunoblotting. Cont, control; IMab-4, intramuscular antibiotic treatment for 4 days; IMab-6, intramuscular antibiotic treatment for 6 days; *, P < 0.05; **, P < 0.01; ***, P < 0.001. n = 3/group.