. 2014 May;82(5):1982–1993. doi: 10.1128/IAI.01199-13

TABLE 1.

Panels of surface markers used to characterize populations of hematopoietic cells^a

Cell type	Marker detected by:
Cell type	FITC	PE	PerCP-Cy5.5	PE-Cy7	APC	APC-Cy7	Pacific Blue
PMNs	CD11b⁺	B220⁻	CD8⁻	CD45⁺	Ly6C⁻	CD115⁻	Ly6G⁺
Monocytes	CD11b⁺	B220⁻	CD8⁻	CD45⁺	Ly6C⁺	CD115⁺	Ly6G⁻
B cells	CD11b⁻	B220⁺	CD8⁻	CD45⁺	Ly6C⁻	CD115⁻	Ly6G⁻
Macrophages	CD11b⁻	CD11c^high	Autofluor⁺	CD45⁺	I-A^k−	CD103⁻	Ly6G⁻
CD11b⁺ DCs	CD11b⁺	CD11c^high	Autofluor⁻	CD45⁺	I-A^k+	CD103⁻	Ly6G⁻
CD103⁺ DCs	CD11b⁻	CD11c^high	Autofluor⁻	CD45⁺	I-A^k+	CD103⁺	Ly6G⁻
CD4⁺ T cells	TCRβ⁺	CD3⁺	CD8⁻	CD45⁺	CD4⁺	TCRγ/δ⁻	Dx5⁻
CD8⁺ T cells	TCRβ⁺	CD3⁺	CD8⁺	CD45⁺	CD4⁻	TCRγ/δ⁻	Dx5⁻
γ/δ T cells	TCRβ⁻	CD3⁺	CD8⁻	CD45⁺	CD4⁻	TCRγ/δ⁺	Dx5⁻
NK cells	CD3⁻	IFN-γ^+/−		CD45⁺	NK1.1⁺
Progenitors				CD31⁺			Ly6C^mid
Peritoneal monocytes		B220⁻	F4/80⁻		CD115⁺
Peritoneal macrophages		B220⁻	F4/80⁺		CD115⁺

See reference 23. Dead cells were excluded from the analysis. Positive markers are in boldface type. CD45 was not included for cells isolated from bone marrow (progenitors) or from peritoneal wash specimens (monocytes and macrophages), as virtually all cells isolated from these sites are of hematopoietic origin. Abbreviations: PMNs, polymorphonuclear cells (neutrophils); DCs, dendritic cells; Autofluor, autofluorescence.