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. 2014 May;82(5):2037–2047. doi: 10.1128/IAI.01530-14

FIG 3.

FIG 3

Growth characteristics of C. trachomatis CtL2 mCh(Gro) strain. (A) Black and white micrographs of confluent HeLa cells infected with a strain of C. trachomatis expressing mCherry under the control of the groESL operon regulatory element [CtL2 mCh(Gro)]. The infected cells were incubated with the indicated concentrations of aTc (0, 2, 20, and 200 ng/ml) at 1 h postinfection and imaged at the indicated times postinfection (24, 48, 72, and 96 h). The channel displaying the mCherry bacteria is shown. (B) Quantification of the numbers of infectious bacteria produced (IFUs/ml) at 48 h, 72 h, and 96 h postinfection (light gray, dark gray, and black bars, respectively) in HeLa cells infected with a strain of C. trachomatis expressing mCherry under the control of the groESL operon regulatory element [CtL2 mCh(Gro)] and incubated in the presence of 0, 2, 20, or 200 ng/ml aTc at 1 h postinfection.