FIG 6.

The massive recruitment of CERT to the inclusion membrane depends on IncD and the PH domain of CERT. (A and C) Confocal fluorescence micrographs of a single plane crossing the middle of inclusions of a C. trachomatis strain, expressing mCherry (red) and IncD3×FLAG (blue) under the control of an aTc-inducible promoter, in HeLa cells expressing wild-type YFP-CERT (YFP-CERT; yellow) (A) or a truncated version lacking the PH domain (YFP-CERT NoPH; yellow) (C). At 1 h postinfection, the infected cells were incubated in the absence (No aTc) or presence (+ aTc) of 20 ng/ml aTc. The cells were fixed at 23 h post-aTc induction, immunostained with anti-FLAG antibodies (IncD3FLAG; blue), and imaged using a confocal microscope. Merged images are shown on the left. Bar, 10 μm. (B and D) Quantifications of the proportions of the inclusion membrane covered by the indicated markers. The quantification method is described in Materials and Methods.