TABLE 1.
E. coli strains and plasmids used in this study
| Strain | Relevant properties | Plasmid | Source or reference |
|---|---|---|---|
| BL21 | F− ompT hsdS (rB− mB−), gal dcm | GE Healthcare | |
| 8751 | Fusion 2b construct, BL21/pfusion-2b | LT192-L-STa13/pET28α | 22 |
| 8752 | Fusion 3b construct, BL21/pfusion-3b | STa13-gly-pro-LT192/pET28α | 22 |
| 8753 | Fusion 4b construct, BL21/pfusion-4b | LT192A1-gly-pro-STa13-LTA2-B/pET28α | 22 |
| 9064 | STa recombinant strain, BL21 | p8835, STa in pUC19 | This study |
| 9355 | 3×STaS3F-dmLT strain, BL21 | p3×STaS3F-dmLT in pET28α | This study |
| 9330 | 3×STaE8Q-dmLT strain, BL21 | p3×STaE8Q-dmLT in pET28α | This study |
| 9357 | 3×STaL9A-dmLT strain, BL21 | p3×STaL9A-dmLT in pET28α | This study |
| 9359 | 3×STaN12I-dmLT strain, BL21 | p3×STaN12I-dmLT in pET28α | This study |
| 9331 | 3×STaN12S-dmLT strain, BL21 | p3×STaN12S-dmLT in pET28α | This study |
| 9361 | 3×STaN12T-dmLT strain, BL21 | p3×STaN12T-dmLT in pET28α | This study |
| 9332 | 3×STaP13A-dmLT strain, BL21 | p3×STaP13A-dmLT in pET28α | This study |
| 9333 | 3×STaP13F-dmLT strain, BL21 | p3×STaP13F-dmLT in pET28α | This study |
| 9363 | 3×STaA14H-dmLT strain, BL21 | p3×STaA14H-dmLT in pET28α | This study |
| 9334 | 3×STaA14Q-dmLT strain, BL21 | p3×STaA14Q-dmLT in pET28α | This study |
| 9335 | 3×STaA14T-dmLT strain, BL21 | p3×STaA14T-dmLT in pET28α | This study |
| 9336 | 3×STaA14V-dmLT strain, BL21 | p3×STaA14V-dmLT in pET28α | This study |
| 9337 | 3×STaT16K-dmLT strain, BL21 | p3×STaT16K-dmLT in pET28α | This study |
| 9374 | 3×STaT16 M-dmLT strain, BL21 | p3×STaT16 M-dmLT in pET28α | This study |
| 9353 | 3×STa-dmLT strain, BL21 | p3×STa-dmLT in pET28α | This study |
| 8955 | Negative control, BL21/pET28α | pET28α | 23 |