TABLE 2.
STa-toxoid forward and reverse (STa-toxoid-F, STa-toxoid-R) PCR primers designed to mutate the STa gene (estA) for STa toxoids which were fused to a modified LT toxoid gene for STa-toxoid-dmLT toxoid fusionsa
| STa toxoid | Primer | Nucleotide sequence (5′-3′) |
|---|---|---|
| 3F | hSTaS3F-F | ATG AAT AGT TTC AAT TAC TGC TGT |
| hSTaS3F-R | ACA GCA GTA ATT GAA ACT ATT CAT | |
| 8Q | hSTaE8Q-F | AAT TAC TGC TGT CAA TTG TGT TGT |
| hSTaE8Q-R | ACA ACA CAA TTG ACA GCA GTA ATT | |
| 9A | hSTaL9A-F | TAC TGC TGT GAA GCA TGT TGT AAT |
| hSTaL9A-R | TGG ATT ACA ACA TGC TTC ACA GCA | |
| 12I | hSTaN12I-F | GAA TTG TGT TGT ATC CCT GCT TGT |
| hSTaN12I-R | ACA AGC AGG GAT ACA ACA CAA TTC | |
| 12S | hSTaN12S-F | GAA TTG TGT TGT AGC CCT GCT TGT |
| hSTaN12S-R | ACA AGC AGG GCT ACA ACA CAA TTC | |
| 12T | hSTaN12T-F | GAA TTG TGT TGT ACC CCT GCT TGT |
| hSTaN12T-R | ACA AGC AGG GGT ACA ACA CAA TTC | |
| 13A | hSTaP13A-F | TTG TGT TGT AAT GCA GCT TGT ACC |
| hSTaP13A-R | GGT ACA AGC TGC ATT ACA ACA CAA | |
| 13F | hSTaP13F-F | TTG TGT TGT AAT TTT GCT TGT ACC |
| hSTaP13F-R | GGT ACA AGC AAA ATT ACA ACA CAA | |
| 14H1 | hSTaA14H-F | TGT TGT AAT CCT CAT TGT ACC GGG |
| hSTaA14H-R | CCC GGT ACA ATG AGG ATT ACA ACA | |
| 14Q | hSTaA14Q-F | TGT TGT AAT CCT CAG TGT ACC GGG |
| hSTaA14Q-R | CCC GGT ACA CTG AGG ATT ACA ACA | |
| 14T | hSTaA14T-F | TGT TGT AAT CCT ACA TGT ACC GGG |
| hSTaA14T-R | CCC GGT ACA TGT AGG ATT ACA ACA | |
| 14V | hSTaA14V-F | TGT TGT AAT CCT GTA TGT ACC GGG |
| hSTaA14V-R | CCC GGT ACA TAC AGG ATT ACA ACA | |
| 16K | hSTaT16K-F | AAT CCT GCT TGT AAG GGG TGC TAT |
| hSTaT16K-R | ATA GCA CCC CTT ACA AGC AGG ATT | |
| 16M | hSTaT16 M-F | AAT CCT GCT TGT ATG GGG TGC TAT |
| hSTaT16 M-R | ATA GCA CCC CAT ACA AGC AGG ATT |
a
Nucleotides that are underlined indicate mutations.