FIG 5.

Invasion of C. sakazakii into Caco-2 cells (4 to 7 days old or 14 to 17 days old) after pretreatment with EGTA. Cell monolayers were pretreated with 5 mM EGTA (or not pretreated) and used for an invasion assay. The data represent the relative percent invasion ± the SD from at least three independent experiments performed in duplicate compared to the invasion of WT to EGTA-untreated Caco-2 cells that were 4 to 7 days old. P values of <0.05 were considered significantly different, and bars with same lowercase letter are not significantly different from one another.