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. 2014 May;82(5):1755–1765. doi: 10.1128/IAI.01397-13

FIG 6.

FIG 6

In vivo animal study. C sakazakii WT and mutants (ΔompA::Kanr, Δinv::Kanr, and ΔompA Δinv) were grown to exponential phase and mixed in a 1:1 ratio (reference strain to test strain) to give four different mixed cultures that were fed (107 CFU) to each group of five (n = 5) rat pups (4 days old) by oral gavage. The pups were then sacrificed at 24 h postinfection. The livers and spleens were removed, homogenized, and plated on BHI1/2 agar and BHI1/2 agar containing 50 μg of kanamycin/ml. The CI score per organ [(the CFU of the reference strain – the CFU of the test strain)/(the CFU of the test strain)] was plotted with the mean CI ± the SD, where a CI of 1.0 indicates no difference in virulence between the reference and test strains. P values of <0.05 were considered significantly different, and bars with same lowercase letter are not significantly different from one another.

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