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. 2014 May;82(5):1755–1765. doi: 10.1128/IAI.01397-13

TABLE 1.

Bacterial strains and plasmids

Strain or plasmid Relevant characteristicsa Source or reference
Strains
    C. sakazakii
        ATCC 29544 Wild-type strain 17
        ES1001 29544 harboring pKD46(Apr) 17
        ES2005 ΔompA 17
        ES2010 Δinv::Kanr This study
        ES2011 Δinv This study
        ES2012 pACYC184-inv This study
        ES2013 ΔompA::Kanr This study
        ES2014 ΔompA Δinv This study
    E. coli
        DH5α λ ϕ80dlacZΔ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK mK) supE44 thi-1 gyrA relA1 17
        Top10 F mcrA Δ(mrr-hsdRMS-mcrBC) 80lacZΔM15 ΔlacX74 recA1 ΔaraD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
Plasmids
    pKD13 oriR6Kγ Apr FRT Kanr FRT 16
    pKD46 oriR101 repA101t,s Apr araBAD pgam-bet-exo 16
    pCP20 oripSC101t,s Apr Cmr cI857λ PRflp 16
    pACYC184 Tetr Cmr; p15A ori 16
    pBAD202/D-TOPO Kanr Invitrogen
    pBADD202-r-inv Harboring C. sakazakii inv gene reverse complement This study
a

Apr, ampicillin resistance; Kanr, kanamycin resistance; Cmr, chloramphenicol resistance; Tetr, tetracycline resistance.

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