TABLE 1.
Strain or plasmid | Relevant characteristicsa | Source or reference |
---|---|---|
Strains | ||
C. sakazakii | ||
ATCC 29544 | Wild-type strain | 17 |
ES1001 | 29544 harboring pKD46(Apr) | 17 |
ES2005 | ΔompA | 17 |
ES2010 | Δinv::Kanr | This study |
ES2011 | Δinv | This study |
ES2012 | pACYC184-inv | This study |
ES2013 | ΔompA::Kanr | This study |
ES2014 | ΔompA Δinv | This study |
E. coli | ||
DH5α | λ− ϕ80dlacZΔ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK− mK−) supE44 thi-1 gyrA relA1 | 17 |
Top10 | F− mcrA Δ(mrr-hsdRMS-mcrBC) 80lacZΔM15 ΔlacX74 recA1 ΔaraD139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
Plasmids | ||
pKD13 | oriR6Kγ Apr FRT Kanr FRT | 16 |
pKD46 | oriR101 repA101t,s Apr araBAD pgam-bet-exo | 16 |
pCP20 | oripSC101t,s Apr Cmr cI857λ PRflp | 16 |
pACYC184 | Tetr Cmr; p15A ori | 16 |
pBAD202/D-TOPO | Kanr | Invitrogen |
pBADD202-r-inv | Harboring C. sakazakii inv gene reverse complement | This study |
Apr, ampicillin resistance; Kanr, kanamycin resistance; Cmr, chloramphenicol resistance; Tetr, tetracycline resistance.