TABLE 2.
Oligonucleotide primers used for PCR amplification and sequencing
| Primer | Sequence (5′–3′)a | Reference or source |
|---|---|---|
| OmpA-F | GTG CTC ATC AAT AGA CCG ACA | 17 |
| OmpA-R | ACT ACA TGC AGC AGA GAA ATT | |
| Inv_ FP | ATG CAT GAG CAA TCC ATA ATG | This study |
| Inv_RP | TTA AAG CAA ATA ATC TCT GGT | |
| Inv_F1 | ATG CTG TCT GGT CTG GCA TCG | This study |
| Inv_R1 | CCA CGT TTA ATC ACT GCT GCG | |
| Inv_F2 | GGG TCA GCG TCA CCT GGA ACG | This study |
| Inv_R2 | CGT CAT ATC CGC CAC CGC TTT | |
| Inv_F3 | CGG TGA TAA CGT GGC GTT GTT | This study |
| Inv_R3 | CAC CGG CAC CGC GGT AAA GAC | |
| Inv_Kan_F | CAT CAT TAA GGA TAC CTG TAG CAC GGG TGT TGA TTG AGA ATT T TGT AGG CTG GAG CTG CTT CG* | This study |
| Inv_Kan_R | ATA CGG AGC CGA AAT CAG GGG AGT TAA TAA GAT GCC ATG A ATT CCG GGG ATC CGT CGA CC* | This study |
| inv HindIII-F | AGA GAG GCA TGC GCT TTC AGA CTC** | This study |
| inv SphI-R | AGA GAG AAG CTT TTA AAG CAA ATA** | This study |
| Inv_pBAD_F | ATG CAT GAG CAA TCC ATA ATG | This study |
| Inv_pBAD_R | CAC CAA GCA AAT AAC TTC TGG TAT TGG T*** | This study |
| Primer_2_F inV | GCA ACA TTG GCT TAG GGG TA | This study |
| Primer_2_R inV | AGC TAT TGG CGG AAA GTT TG | This study |
a
*, Nucleotide sequences that originated from the C. sakazakii ATCC 29544 inv gene are shown in italics, and those from pKD13 are underlined; **, artificially added restriction enzyme recognition sites are underlined; ***, an artificially added four-base sequence is underlined.