TABLE 3.
Specimens positive for Plasmodium malariaea
| Specimen no. | Diagnosed species | Microscopy | Parasitemia (%) | Binaxb |
Genus qPCR, current assay (CT) | P. falciparum qPCR, current assay (CT) | P. vivax qPCR, current assay (CT) | P. malariae qPCR, current assay (CT) | P. ovale qPCR, current assay (CT) | P. malariae qPCR, new assay (CT) | P. ovale qPCR, new assay (CT) | 100% sequence homolog in blast | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| T1 | T2 | ||||||||||||
| 34 | P. malariae | P. malariae | <0.1 | − | + | 31.1 | − | − | 31.8 | − | 27.3 | − | P. malariae |
| 35 | P. malariae | P. malariae | 0.2 | − | + | 25.9 | − | − | 26.5 | − | 22.7 | − | P. malariae |
| 36 | P. malariae | P. malariae | <0.1 | − | + | 29.6 | − | − | 29.6 | − | 26.1 | − | P. malariae |
| 37 | P. malariae | P. malariae | <0.1 | − | + | 28.1 | − | − | 28.3 | − | 25.0 | − | P. malariae |
| 38 | P. malariae | P. malariae | <0.1 | − | + | 27.4 | − | − | 27.5 | − | 24.6 | − | P. malariae |
| 39 | P. malariae | Psp | <0.1 | − | − | 34.5 | − | − | 34.3 | − | 30.8 | − | P. malariae |
| 40 | P. malariae | Psp | <0.1 | − | − | 33.8 | − | − | 34.4 | − | 31.0 | − | P. malariae |
| 41 | P. malariae | P. malariae | <0.1 | NDc | ND | 28.8 | − | − | 29.0 | − | 25.7 | − | P. malariae |
| 42 | P. malariae | P. malariae | <0.1 | − | + | 29.3 | − | − | 29.3 | − | 26.0 | − | P. malariae |
| 43 | P. malariae | P. malariae | <0.1 | − | − | 29.6 | − | − | 30.6 | − | 26.7 | − | P. malariae |
| 44 | P. malariae | P. malariae | <0.1 | − | + | 26.5 | − | − | 26.2 | − | 22.9 | − | P. malariae |
| 45 | P. malariae | P. malariae | 0.2 | − | + | 29.8 | − | − | 30.2 | − | 25.8 | − | P. malariae |
| 46 | P. malariae | P. malariae | <0.1 | − | + | 26.3 | − | − | 26.5 | − | 24.2 | − | P. malariae |
| 47 | P. malariae | P. malariae | <0.1 | − | + | 27.9 | − | − | 28.2 | − | 24.7 | − | P. malariae |
| 48 | P. malariae | P. malariae | 0.2 | − | + | 25.4 | − | − | 24.9 | − | 23.2 | − | P. malariae |
a
P. malariae-positive specimens (n = 15) used in this study. Results from various diagnostic assays are shown. Species unidentifiable by microscopy are indicated as Psp. Species identification was based on concordance of 2 out of 3 species-specific tests, microscopy, qPCR, and BLAST sequence homology. Real-time quantitative PCR (qPCR) tests were run and compared for all 48 specimens with the protocol previously published by Khairnar et al. (current assay) (3) and a new assay with primer/probe sequences and concentrations designed in this study.
b
Binax T1, Plasmodium falciparum; Binax T2, Pan Plasmodium.
c
ND, not determined.