TABLE 4.

P. malariae and P. ovale duplex qPCR primer/probe sequences and final concentrations^a

Primer/probe	Sequenceb	Final concn (nM)
P. malariae forward primerc	CCGACTAGGTGTTGGATGATAGAGTAAA	200
P. malariae probec	6FAM-CTATCTAAAAGAAACACTCAT-MGBNFQ	80
P. ovale forward primer D2	CCRACTAGGTTTTGGATGAAAVRTTTTT	200
P. ovale probe D	VIC-CRAAAGGAATTYTCTTATT-MGBNFQ	160
Plasmodium reverse primerc	AACCCAAAGACTTTGATTTCTCATAA	200

^aP. ovale degenerate (D) primer and probe were designed based on sequence heterogeneity found in this study.

^bR = A or G; Y = C or T; V = A or C or G. Underlining indicates degenerate nucleotides.

^cSequences as previously published (4).