TABLE 1.
Sequences of primers and probes designed for the multiplex real-time PCR assay
| Species and its primers and probe | Sequencea |
|---|---|
| Pneumocystis jirovecii | |
| OliPJMB1 (f) | 5′-CCCTAGTGTTTTAGCATTTTTC-3′ |
| OLIPJMB2 (r) | 5′-CTGCAATTCACACTACTTATCG-3′ |
| Probe PJ-MB1 | 5′-HEX-CGCGATACCTTTGGCGAGGCAAGCAAATCGCG-BHQ1-3′ |
| Histoplasma capsulatumb | |
| Cryptococcus neoformans | |
| OLI CRYPTO 1 2 (f) | 5′-CCTGTTGGACTTGGATTTGG-3′ |
| OLI CRYPTO 2 (r) | 5′-AGCAAGCCGAAGACTACC-3′ |
| Probe MB CRYPTO | 5′-Cyan 500-CGCGATCATTACGCCGGGCTGACAGGTAATCAGATCGCG-BHQ1-3′ |
| Jellyfish (internal control) | |
| Oli1-icjf1 (f) | 5′-GCCTGGTGCAAAAATTGCTTATC-3′ |
| Oli1-icjf2 (r) | 5′-CTAAGACAAGTGTGTTTATGGTATTG-3′ |
| Probe CIJF-MB | 5′-Cy5-CGCGATGCTGTTCTTCCGCCACTTCCAATCGCG-BHQ2-3′ |
a
BHQ1, black hole quencher 1.
b
Sequences for the primers and probe for H. capsulatum are included in patent PCT/ES2009070340.