TABLE 3.
Summary of control populations used to determine background colonization levels and the specificity of the Mrt-PCR assay
| Population | Control sample no. | Specimen | PCR result | Species causing IFI |
|---|---|---|---|---|
| HIV+ patients from areas of endemicity | 1 | Bone narrow | Negative | |
| 2 | Biopsy | Negative | ||
| 3 | Biopsy | Negative | ||
| 4 | Bone narrow | Negative | ||
| 5 | Whole Blood | Negative | ||
| 6 | Whole Blood | Negative | ||
| HIV+ patients from areas of endemicity and with viral or bacterial pneumonia | 7 | BAL | Negative | |
| 8 | BAL | Negative | ||
| 9 | BAL | Negative | ||
| 10 | Biopsy | Negative | ||
| HIV+ patients with invasive fungal infection caused by other fungal species | 11 | Biopsy | Negative | Lichtheimia corymbifera |
| 12 | Biopsy | Negative | Rhizomucor pusillus | |
| 13 | BAL | Negative | Cryptococcus laurentii | |
| 14 | BAL | Negative | Cryptococcus humicola | |
| 15 | BAL | Negative | Aspergillus flavus, Penicillium spp., and Cryptococcus albidus | |
| Non-HIV-infected patients with invasive fungal infection | 16 | Biopsy | Negative | Coccidioides immitis |
| 17 | Biopsy | Negative | Scedosporium apiospermum | |
| 18 | Biopsy | Negative | Bipolaris spp. | |
| 19 | BAL | Negative | Aspergillus fumigatus | |
| 20 | BAL | Negative | Aspergillus fumigatus | |
| 21 | BAL | Negative | Aspergillus fumigatus | |
| 22 | BAL | Negative | Aspergillus fumigatus | |
| 23 | BAL | Negative | Aspergillus fumigatus | |
| 24 | Ascitic fluid | Negative | Aspergillus fumigatus | |
| 25 | Cerebrospinal fluid | Negative | Aspergillus fumigatus | |
| 26 | BAL | Negative | Aspergillus fumigatus | |
| 27 | Biopsy | Negative | Aspergillus fumigatus | |
| 28 | Biopsy | Negative | Aspergillus fumigatus | |
| 29 | BAL | Negative | Aspergillus fumigatus |