FIG 1.

Detection of ileS2 using PCR. Lanes 1 to 3 include PCR products amplified with mupA and mupB primers (9). Lanes 4 to 6 include PCR products amplified with M1 and M2 primers (10). The molecular size marker used in lanes 1 and 8 was a 100-bp DNA ladder (Invitrogen, Grand Island, NY). Numbers at left are molecular sizes in bp. Template DNA used for PCR was plasmid DNA from isolate 39-045 (lanes 2 and 5) or genomic DNA from ATCC 29213 (lanes 3 and 6). Water was substituted for DNA in lanes 4 and 7.