FIG 2.
Detection of the ileS2-IS257 junctions in isolate 39-045 using ileS2-5′, IS257F, ileS2-3′, and IS257R primers (11). Primer pairs for each reaction were as follows: PCR 1, ileS2-5′ and IS257F; PCR 2, ileS2-5′ and IS257R; PCR 3, ileS2-3′ and IS257F; and PCR 4, ileS2-3′ and IS257R. Lanes 2, 6, and 10 are products from PCR 1; lanes 3, 7, and 11 are products from PCR 2; lanes 4, 8, and 12 are products from PCR 3; lanes 5, 9, and 13 are products from PCR 4; and lanes 1 and 14 are a 1-kb DNA ladder (Invitrogen, Grand Island, NY). Template DNA in lanes 2 to 5 is plasmid DNA from isolate 39-045. Template DNA in lanes 6 to 9 is genomic DNA from ATCC 29213 used as a negative control. In lanes 10 to 13, water was substituted for template DNA as a negative control. No positive controls were available. Numbers at left are sizes in bases.